Kinetic Constants of the Human Renin and Human Angiotensinogen Reaction
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چکیده
منابع مشابه
Kinetic Constants of the Human Renin and Human Angiotensinogen Reaction
The rate of formation of angiotensin was investigated for the reaction of angiotensinase-free human renin, at various states of purity, with angiotensinogen, in pooled, angiotensinase-free human serum. The velocity constant of this reaction and the Michaelis constant of the rerun-substrate complex were determined. The concentration of angiotensinogen and of endogenous renin in human serum was a...
متن کاملKinetic constants of the human renin and human angiotensinogen reaction.
The rate of formation of angiotensin was investigated for the reaction of angiotensinase-free human renin, at various states of purity, with angiotensinogen, in pooled, angiotensinase-free human serum. The velocity constant of this reaction and the Michaelis constant of the rerun-substrate complex were determined. The concentration of angiotensinogen and of endogenous renin in human serum was a...
متن کاملSer84 of human renin contributes to the biphasic pH dependence of the renin-angiotensinogen reaction.
The pH dependence of the reaction of various renins was investigated using sheep angiotensinogen as a substrate. Human renin showed two separate peaks, but rat and mouse Ren1 renins showed one peak with a shoulder. A comparison of the predicted subsite residues of human renin with those of rat and mouse Ren1 renins revealed that Arg82, Ser84, Thr85, Ala229, and Thr312 are unique in the human se...
متن کاملAppropriate regulation of renin and blood pressure in 45-kb human renin/human angiotensinogen transgenic mice.
The renin-angiotensin system is normally subject to servo control mechanisms that suppress plasma renin levels in response to increased blood pressure and increase plasma renin levels when blood pressure falls. In most species, renin is rate limiting, and angiotensinogen circulates at a concentration close to the Km, so varying the concentration of either can affect the rate of angiotensin form...
متن کاملComplementation of reduced survival, hypotension, and renal abnormalities in angiotensinogen-deficient mice by the human renin and human angiotensinogen genes.
The aim of this study was to determine whether elements of the human renin-angiotensin system (RAS) could functionally replace elements of the mouse RAS by complementing the reduced survival and renal abnormalities observed in mice carrying a gene-targeted deletion of the mouse angiotensinogen gene (mAgt). Double transgenic mice containing the human renin (HREN) and human angiotensinogen (HAGT)...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 1967
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.20.1.45